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KMID : 0361020150580020095
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Korean Journal of Otolaryngology - Head and Neck Surgery
2015 Volume.58 No. 2 p.95 ~ p.100
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Factors Affecting the Expansion of Chondrocytes Harvested from Rabbit Auricle and Nasal Septum
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Jin Kyung-Suk
Park So-Young
Kim Byung-Guk
Choi Ha-Na
Jung Young-Hoon
Oh Jeong-Hoon
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Abstract
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Background and Objectives: In tissue engineering of the cartilage, cellular expansion is an essential step for creating useful amounts of tissue required. Many variables such as media composition, growth factors and cell seeding density have been utilized to stimulate the ability of cellular proliferation and to reduce the number of expansion in monolayer. The object of this study was to evaluate the effects of human serum (HS) and rabbit serum (RS) on cellular proliferation of auricular and nasal septal chondrocytes of the rabbit.
Subjects and Method: Auricular and nasal septal chondrocytes of the New Zealand White Rabbit were expanded in monolayer in medium supplemented with 2% RS, 5% RS, 10% RS, or 10% HS with or without adding of growth factors. The effect of the supplements on the cellular proliferation was compared.
Results: The proliferation yield of chondrocytes grown in RS was comparable to that with HS. Both serums results in increased proliferation; however, the amount of cellular proliferation was not correlated with the concentration of the serum. The proliferation rate of nasal septal chondrocytes was higher than that of auricular chondrocytes.
Conclusion: Both HS and RS resulted in the increased proliferation of rabbit auricular and nasal septal chondrocytes. The results show that the determination of appropriate types and concentrations of serum is required for cell expansion in constructing tissue-engineered cartilage.
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KEYWORD
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Auricle, Chondrocyte, Nasal septum, Proliferation, Serum
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